Not known Factual Statements About flow cytometer
Not known Factual Statements About flow cytometer
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Information-enriched characterization of cell populations. Morphological data from photos can insert to the richness of flow cytometry info. Such as, the determine displays an normally standard apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in Every single populace to reveal morphologically distinct options. These insights could not have been obtained from multiplex staining on your own.
No matter whether you’re a newbie or a professional, examine our sources to progress your investigation and knowledge in flow cytometry.
(B) Uncooked 264.7 macrophages are sometimes annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The amount of singlets readily available for Assessment raises once the consumer trains and employs a more exact model.
Find new insights into your experimental info by visualizing populations utilizing combined fluorescence and morphology information. Our software program interface wizards permit downsampling, concatenation (merged files) and dimensionality tools that happen to be created in the software program for an intuitive, streamlined info Examination knowledge.
"An image just isn't pretty really worth one thousand dots, but it really genuinely helps to see images. It is great to check out photographs that correlate with staining. By way of example, AnnexinV-positive cells which have been morphologically distinct than healthy cells, or CD14+ monocytes which have been greater and even more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it truly is illuminating to determine how many doublets slip via conventional singlet gates, and good to validate the position of surprising double-beneficial activities in my Examination."
Pair your Attune flow cytometer with an autosampler for greater workflow efficiency, and add robotic integration for maximum throughput.
Steady image good quality even at substantial throughput Acoustic focusing in addition to a substantial-speed camera Mix to picture these T cells persistently at lower or superior flow rates. Quickly alter emphasis and digicam options to fulfill experimental specifications.
Early signs of this contamination had at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also means that you can use morphological capabilities to discover exciting subpopulations that would not be clear from flow cytometry info on your own.
Consumers can perspective celebration illustrations or photos in actual-time to verify sample quality, improve protocols, and document the morphology of every cell populace identified for analysis. Imaging seize also Positive aspects from acoustic concentrating, which helps you to posture the cells to ensure that a sharp, centered impression is obtained.
AI-driven automatic image Assessment Together with the Attune Cytometric Computer software interprets occasion functions into unique morphology parameters which can be coupled with check here normal fluorescence and scatter parameters to expose hugely reproducible population modifications and one of a kind subsets.
We Earlier demonstrated the strength of imaging Car or truck-T/Ramos mobile interactions. Allow’s evaluate just the population of greatest desire, the double positive functions, to learn more. We are able to now use extended morphological parameters (in this article, circularity and skewness of intensity) to additional study the attributes of such populations and refine gating on these situations, growing data robustness.
Designed-in dimensionality reduction resources improved resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to substantiate envisioned populace resolution in lysed whole human blood. User can see situations from Each individual cluster for visual affirmation of mobile phenotypes.
Versatile payment approach from Agilent Money Remedies help you receive the essential instrumentation you may need for fast venture, or to expand your lab.
Gating on these extended parameters lets you speedily and properly determine populations of interest and make sure gating tactic with little if any guide assessment. The Attune computer software provides access to condition-of-the-art equipment Understanding Investigation tools within just an expertly made person interface, which makes it exceptionally simple to operate.
The Attune Xenith cytometer is usually a high-parameter spectral analyzer, offering both spectral unmixing and conventional flow capabilities for extensive and versatile cell analysis.
The Attune CytPix Flow Cytometer permits users to visualize samples all through acquisition, verify the precision of gating methods, and aid outcomes with dependable documentation for publication uses.
What appeared to be a homogeneous population is really more numerous—an insight that ought to be regarded when interpreting outcomes.